- Open Access
Reliability and validity of the Chinese version of the Psoriasis Disability Index (PDI) in Chinese patients with psoriasis
- Zehui He†1,
- Chuanjian Lu†2,
- Aihua Ou3,
- Jiqian Fang1Email author,
- Dongmei Wang2,
- Jingwen Deng2,
- Zhongzhao Zhang2 and
- Jingjie Yu2
© He et al; licensee BioMed Central Ltd. 2012
Received: 12 January 2012
Accepted: 13 April 2012
Published: 13 April 2012
The Psoriasis Disability Index (PDI) is a widely used instrument to measure the impact of psoriasis on patients. There has not been psychometric evaluation of the Chinese version of PDI. The aim of this study was to evaluate its reliability and validity among Chinese patients with psoriasis.
A multi-center, cross-sectional study was conducted at 9 hospitals including patients aged 18 years and over. Reliability was determined by internal consistency using Cronbach’s alpha. Validity was assessed through convergent validity and known groups validity. Dimensionality of the PDI was examined by exploratory factor analysis in working patients and nonworking patients respectively.
In all, 831 patients were studied. Internal consistency of the PDI was satisfactory. Cronbach’s alpha coefficient was 0.91 for the total score and over 0.70 for each subscale of the PDI. Evidence of convergent validity of the PDI was proved by excellent and moderate to good correlations with the Dermatology Life Quality Index (DLQI) and four subscales of the Short Form-36 (SF-36) (role-physical, bodily pain, social functioning, and role-emotional): r = 0.51-0.78. Known groups validity was confirmed that the PDI score discriminated well among patients with different severity of psoriasis. The dimensionality of the PDI was determined by the presence of two-factor structure for working patients and three-factor structure for nonworking patients which accounted for 57.3% and 62.3% of the variance respectively.
The Chinese version of the PDI is a reliable and valid instrument to assess the impact of psoriasis on patients’ lives and could be used in future quality of life assessment of Chinese patients with psoriasis.
Psoriasis is a chronic, systemic, and incurable skin condition characterized by the skin signs of thick scaling red plaques with itching and arthritis. It has been proved that psoriasis is associated with risk of cardiovascular disease, diabetes, overweight/obesity and depression [1–5]. Psoriasis can has profound effects on patients’ daily living and functioning [6–8]. While measurements of clinical severity separately using tools such as the Psoriasis Area and Severity Index (PASI)  are not sufficient and may not reflect patients’ own perceptions of the impairment due to psoriasis, it is important to assess the impact of psoriasis on patients’ physical condition, self-perception, and social life [10, 11]. Health-Related Quality of Life (HRQOL), covering almost all these parameters, is therefore increasingly part of the clinical research and practice. HRQOL assessment can provide valuable information that helps make clinical decision and select suitable health care programmes [12, 13].
There has been a wide variety of generic, dermatology and psoriasis-specific instruments used for the assessment of HRQOL of psoriasis patients [14–18]. However, the reliability and validity of these instruments are not fully known [19–21]. The reliability and validity are the major evaluations of instruments’ performance of reflecting concepts or ideas such quality of life (QOL) in a study population . Evaluating the characteristics of instruments used to measure patients’ perceptions is important in clinical health care and decision making.
The Psoriasis Disability Index (PDI) , as a psoriasis-specific instrument, was one of the attempts to measure and quantify the impact of psoriasis on patients’ daily lives . It has been used internationally for almost 20 years and has been translated into at least 13 languages . The reliability and validity of the PDI have been evaluated using different languages and in different psoriasis populations. However, the Chinese version of PDI has not been formally validated in Chinese patients with psoriasis. In China, HRQOL research has made a remarkable progress in patient populations , but studies on patients with psoriasis at a population level are limited. One of the main reasons is a lack of suitable instruments in Chinese.
In this study, permission was sought and thereby given from the developers of the PDI. Then we used psychometric methods to evaluate the reliability and validity of the Chinese version of PDI in patients with psoriasis.
Materials and methods
Study design and subjects
A multi-center, cross-sectional study of patients with psoriasis in 9 large hospitals was performed from November 2010 to April 2011. For a representative sample of Chinese patients with psoriasis, outpatients and inpatients with psoriasis were recruited consecutively in dermatological clinics of the 9 hospitals located in different geographical regions of China. In the north and south of China, respectively two hospitals participated in the study. In the east and middle of China, respectively one hospital participated in the study. In the west of China, three hospitals participated in the study. Inclusion criteria for this study were: (1) a clinical confirmed psoriasis diagnosis; (2) age 18 years or older; and (3) willingness to provide consent to participate. Patients were excluded if they had a severe mental illness.
After the purpose and the contents of the study had been fully explained to the subjects, written informed consent was obtained. Then all participants were asked to fill out the questionnaires according to their own feelings and opinions. Those who have difficulties in completing questionnaires by themselves finished the questionnaires with the help of trained investigators, who have been trained that every answer should be solely based on participant’s own response. Completed questionnaires were collected as soon as they were finished.
This study was approved by the Ethics Committee of the Guangdong Provincial Hospital of Traditional Chinese Medicine.
The measurement tools consisted of PDI, Dermatology Life Quality Index (DLQI), Health Survey Short Form (SF-36) and PASI. The DLQI and SF-36 were used to evaluate the convergent validity of the PDI. The PASI, frequently as an assessment of the severity of psoriasis, was used to evaluate the known groups validity of the PDI.
Psoriasis disability index (PDI)
The PDI, developed by Finlay and Coles, concerns the functional lifestyle disabilities caused by psoriasis . It contains 15 items with 5 subscales: daily activities, work, personal relations, leisure, and treatment. All items are rated on a 4-point scale, with responses of “not at all”, “a little”, “a lot”, and “very much” scored 0, 1, 2, and 3, respectively. Item scores are summed to yield a total score (range: 0–45) with higher score indicating greater limitations experienced because of psoriasis. Particularly, the PDI has a possible 5 work items of which only 3 items need to be responded. Respondents who are working (either full- or part-time) respond to item 6a, 7a, and 8, whereas respondents who are not working respond to items 6b, 7b, and 8. When one item of the PDI was not responded, it was scored “0”. When two or more items of the PDI were not responded, the questionnaire was excluded from the analysis.
Dermatology life quality index (DLQI)
The DLQI, developed by Finlay and Khan, assesses the QOL impact of skin disease . Comprising 10 items, the DLQI has been used internationally for more than 15 years and translated into more than 80 international languages [25, 26]. Each item is scored on a 4-point scale (range 0–3). The final score on the questionnaire is the sum of the score of each item (range 0–30). Higher scores indicate worse QOL. Its Chinese version has been evaluated and shown good psychometric properties .
Short form-36 (SF-36)
The SF-36 is a measure of health status and is commonly used in clinical and health services research . Its Chinese version has been tested for psychometric properties . It consists of 8 subscales: physical function (PF), role-physical (RP), body pain (BP), general health (GH), vitality (VT), social functioning (SF), role-emotional (RE), and mental health (MH). The scores of the 8 subscales are calculated according to the scoring algorithm of the SF-36 user’s manual. The higher score, the better health. .
Psoriasis area and severity index (PASI)
The PASI assessed both intensity and extent of the psoriatic plaques separately for four anatomical regions (head, trunk, upper and lower extremities) by the physician. The PASI score ranges from 0 (no psoriasis) to 72 (very severe psoriasis), and it was normally regrouped into three categories implying three severity levels of psoriasis: PASI 0–7 (mild severity), PASI 7–12 (moderate severity), and PASI 12–72 (severe severity) .
Furthermore, demographic and psoriasis-specific variables such as age, gender, marital status, education, co-existing chronic disease, family history of psoriasis, duration, and clinical type of psoriasis, were reported.
For each subscale of the PDI, the floor and ceiling effects were assessed. If more than 20% of the patients reported lowest or highest possible score, the floor or ceiling effects exist . Cronbach’s alpha and item-total correlations were determined to assess the internal consistency. Construct validity, which hypothesizes a scale measure or correlate with the theorized psychological scientific construct that it purports to measure, was assessed by convergent validity and known groups validity. Convergent validity assessed the degree to which the PDI was similar to (converged on) other measures that it should theoretically be associated to. Spearman’s rank correlation coefficient was used to assess correlations between the PDI and the DLQI, the SF-36, which are the two potential measures that assess similar underlying phenomenon as the PDI. Known groups validity tested the ability of the PDI to discriminate between groups that differed in the severity of psoriasis (assessed by PASI). The Kruskal-Wallis test was used to test for statistical differences between the groups. Dimensionality of the PDI was analyzed for patients who were working and not working separately because the work items of the PDI were different for working and nonworking patients. Exploratory factor analysis was used by means of principal component analyses. Oblique rotation (promax) was performed according to the supposed correlations between the factors.
All statistical analysis was performed using SPSS 17.0 (SPSS Inc., Chicago, IL, USA).
In total, 884 patients participated in this study. Out of these, 9 patients were less than 18 years old. Further, 44 patients were excluded because they had two or more missing answers on the PDI. Hence, the analysis was carried out on a total of 831 patients (94%).
Demographic and clinical characteristics of the subjects
Total sample(N = 831)
North (N = 172)
South (N = 180)
East (N = 96)
West (N = 284)
Middle (N = 99)
Marital status (%)
Married or living with partner
Level of education (%)
Higher than college
Other chronic disease (%)
Family history of psoriasis (%)
Duration in years mean (SD)
PASI score mean (SD)
Descriptive information and Cronbach’s alpha of the PDI total scale and its subscales
No. of items (score range)
Corrected item-total correlation
English version b
The means, standard deviations, percentiles, corrected item-total correlations, and Cronbach’s alpha coefficients of the PDI and its subscales are presented in Table 2. The subscales of PDI showed Cronbach’s alpha coefficients above 0.7, and the item-total correlation ranged from 0.32 to 0.74, which suggested an adequate internal consistency of the PDI [32, 33].
Spearman’s rank correlation coefficients between the total PDI score and the DLQI, SF-36 scores
r s (P)
0.78 a (<0.001)
−0.42 c (<0.001)
−0.58 b (<0.001)
−0.51 b (<0.001)
−0.34 c (<0.001)
−0.40 c (<0.001)
−0.65 b (<0.001)
−0.58 b (<0.001)
−0.45 c (<0.001)
Known groups validity
Dimensionality of the PDI
Exploratory factor analysis of the PDI
Patients who were working (n = 607)
Patients who were not working (n = 224)
(eigenvalue = 7.46)
(eigenvalue = 1.13)
(eigenvalue = 7.08)
(eigenvalue = 1.21)
(eigenvalue = 1.06)
1. House/garden work
2. Different clothes
3. Change/wash clothes
4. Hairdresser problem
5. More baths
6a. Time off work
7a. Inhibit work
6b. Less activity
7b. Different activity
8. Career affected
9. Sexual difficulties
10. Social relations
11. Social activities
12. Sport difficulties
13. Communal changing
14. More smoke/drink
15. Home messy/untidy
As it is increasingly important to assess the impact of psoriasis on the lives of patients, this study adapted an original English and widely used scale PDI to Chinese psoriasis patients. The Chinese version of the PDI was proved to have good reliability and validity by the data presented here.
Response distribution of the PDI
A moderate percentage of patients were likely to response “Not at all” affected by psoriasis for the work, personal, and treatment subscales of the PDI. Comparing to the large floor effects conducted in a US study (>49% except daily activities), the floor effects of the Chinese version of PDI were smaller while both ceiling effects were small . The mild floor effects might suggest that some items of the PDI provide limited information on the disabilities caused by psoriasis, especially for those with low level of disabilities and severities.
Reliability and validity of the PDI
The internal reliability estimated by Cronbach’s alpha exceeded 0.7 in the total scale and all subscales of the PDI, which indicated a good reliability of the instrument. Similar results have been found in other language versions of the PDI [32, 35, 36]. Regarding the validity, excellent and moderate to good correlations with the DLQI and certain subscales of the SF-36 added to available evidence of convergent validity for the PDI. The English and Norwegian versions of the PDI have also proved their convergent validity [32, 35]. The results of known groups comparison indicated the discriminate ability of the PDI was good enough to distinguish the patients with different severities of psoriasis, which was consistent with previous studies .
Dimensionality of the PDI
The items of PDI were grouped into five subscales based on common sense when the PDI was developed . However, the original structure could not be confirmed by factor analysis , and no consensus exists regarding the number of factors composing this scale. It has been observed that three factors labeled physical, social, and hygienic disabilities respectively could underlie the disabilities construct in the Norwegian version . Our results confirmed the two factors conception of the PDI for the working patients, one concerning work-related disabilities, the other concerning hygiene and embarrassment, which was consistent with the results obtained in a US sample . For nonworking patients, our results indicated a similar factor structure as those for working patients, which was not consistent with the extraction of one-factor structure in the US sample, however . The differences between present results and the results in the US sample might be partly due to differences in sample characteristics. The multidimensionality of the PDI documented by the existing studies could compromise the validity of the total score of PDI when it is used for measurement of impact of psoriasis.
Strengths and limitations
This study is important as being the first study to evaluate reliability and validity of the Chinese version of PDI based on a relatively large sample of Chinese patients with psoriasis. The results presented here provide reasonable evidence to show that the Chinese version of PDI has good reliability and validity. There are some limitations, however. Because the PDI was administrated once to the subjects, test-retest reliability, responsiveness to change, and minimal clinical important difference (MCID) were not evaluated. The differential item functioning (DIF) among patients with different demographic variables and cultural background was not evaluated. Enrolled in large hospitals only, the study sample can not be regarded as representative of all Chinese patients with psoriasis. Moreover, there were nearly 30% sample subjects suffering other chronic disease. The reliability and validity of the PDI in these patients need further studies.
The Chinese version of PDI is a reliable and valid instrument and can be used to assess patient-reported impact of psoriasis. It is expected to help improve the QOL assessment of Chinese patients with psoriasis because until now there have been few instruments to measure psoriasis-specific QOL in China. The results are comparable to those of the original English version and translated versions in other countries. Therefore, international cooperative research could use the scale to measure QOL in patients with psoriasis.
ZH contributed to the conception and interpretation of the statistical analysis, and drafted the manuscript. CL contributed to the design of the study, training interviewers and drafting the manuscript. AO conducted the statistical analysis, contributed the interpretation of data. JF contributed to the conception and design of the study, and revised the manuscript. DW, JD, ZZ, and JY contributed to the acquisition of data. All authors read and approved the final manuscript.
We are grateful to Professor Andrew Finlay for permission to use the Chinese version of PDI. This study was funded by the Financial Industry Technology Research and Development Program of Guangdong Province (201105). We thank the investigators of the nine following hospitals: North of China: Beijing Traditional Chinese Medicine Hospital of Capital Medical University, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine; South of China: Guangdong Provincial Hospital of Traditional Chinese Medicine, First Affiliated Hospital of Sun Yat-sen University; East of China: Longhua Hospital of Shanghai University of Traditional Chinese Medicine; West of China: Yunnan provincial Hospital of Traditional Chinese Medicine, Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University; Middle of China: People’s Liberation Army 195 Hospital.
- Griffiths C, Barker JN: Pathogenesis and clinical features of psoriasis. Lancet 2007, 370: 263–271. 10.1016/S0140-6736(07)61128-3View ArticlePubMedGoogle Scholar
- Naldi L, Mercuri SR: Epidemiology of comorbidities in psoriasis. Dermatol Ther 2010, 23: 114–118. 10.1111/j.1529-8019.2010.01304.xView ArticlePubMedGoogle Scholar
- Neimann AL, Shin DB, Wang X, Margolis DJ, Troxel AB, Gelfand JM: Prevalence of cardiovascular risk factors in patients with psoriasis. J Am Acad Dermatol 2006, 55: 829–835. 10.1016/j.jaad.2006.08.040View ArticlePubMedGoogle Scholar
- Zhang C, Zhu KJ, Zheng HF, Cui Y, Zhou FS, Chen YL, Tang XF, Li M, Zhang FY, Fan X, Zuo XB, Yang S, Sun LD, Zhang XJ: The effect of overweight and obesity on psoriasis patients in Chinese Han population: a hospital-based study. J Eur Acad Dermatol Venereol 2010, 25: 87–91.View ArticleGoogle Scholar
- Schmitt JM, Ford DE: Role of depression in quality of life for patients with psoriasis. Dermatology 2007, 215: 17–27. 10.1159/000102029View ArticlePubMedGoogle Scholar
- Krueger G, Koo J, Lebwohl M, Menter A, Stern RS, Rolstad T: The impact of psoriasis on quality of life: results of a 1998 National Psoriasis Foundation patient-membership survey. Arch Dermatol 2001, 137: 280–284.PubMedGoogle Scholar
- Stern RS, Nijsten T, Feldman SR, Margolis DJ, Rolstad T: Psoriasis is common, carries a substantial burden even when not extensive, and is associated with widespread treatment dissatisfaction. J Invest Dermatol Symp Proc 2004, 9: 136–139. 10.1046/j.1087-0024.2003.09102.xView ArticleGoogle Scholar
- Zachariae R, Zachariae H, Blomqvist K, Davidsson S, Molin L, Mørk C, Sigurgeirsson B: Quality of life in 6479 Nordic patients with psoriasis. Br J Dermatol 2002, 146: 1006–1016. 10.1046/j.1365-2133.2002.04742.xView ArticlePubMedGoogle Scholar
- Fredriksson T, Pettersson U: Severe psoriasis-oral treatment with a new retinoid. Dermatologica 1978, 157: 238–244. 10.1159/000250839View ArticlePubMedGoogle Scholar
- Krueger GG, Feldman SR, Camisa C, Duvic M, Elder JT, Gottlieb AB, Koo J, Krueger JG, Lebwohl M, Lowe N, Menter A, Morison WL, Prystowsky JH, Shupack JL, Taylor JR, Weinstein GD, Barton TL, Rolstad T, Day RM: Two considerations for patients with psoriasis and their clinicians: What defines mild, moderate, and severe psoriasis? What constitutes a clinically significant improvement when treating psoriasis? J Am Acad Dermatol 2000, 43: 281–285. 10.1067/mjd.2000.106374View ArticlePubMedGoogle Scholar
- Sampogna F, Sera F, Abeni D: IDI Multipurpose Psoriasis Research on Vital Experiences (IMPROVE) Investigators: Measures of clinical severity, quality of life, and psychological distress in patients with psoriasis: a cluster analysis. J Invest Dermatol 2004, 122: 602–607. 10.1046/j.0022-202X.2003.09101.xView ArticlePubMedGoogle Scholar
- Chren MM, Weinstock MA: Conceptual issues in measuring the burden of skin diseases. J Invest Dermatol Symp Proc 2004, 9: 97–100. 10.1111/j.1087-0024.2004.09119.xView ArticleGoogle Scholar
- Williams HC: Applying trial evidence back to the patient. Arch Dermatol 2003, 139: 1195–1200. 10.1001/archderm.139.9.1195PubMedGoogle Scholar
- Finlay AY, Khan GK: Dermatology Life Quality Index (DLQI) - a simple practical measure for routine clinical use. Clin Exp Dermatol 1994, 19: 210–216. 10.1111/j.1365-2230.1994.tb01167.xView ArticlePubMedGoogle Scholar
- Finlay AY, Coles EC: The effect of severe psoriasis on the quality of life of 369 patients. Br J Dermatol 1995, 132: 236–244.View ArticlePubMedGoogle Scholar
- Chren MM, Lasek RJ, Quinn LM, Mostow EN, Zyzanski SJ: Skindex, a quality-of-life measure for patients with skin disease: reliability, validity, and responsiveness. J Invest Dermatol 1996, 107: 707–713. 10.1111/1523-1747.ep12365600View ArticlePubMedGoogle Scholar
- Morgan M, McCreedy R, Simpson J, Hay RJ: Dermatology Quality of Life Scales-a measure of the impact of skin disease. Br J Dermatol 1997, 136: 202–206. 10.1111/j.1365-2133.1997.tb14896.xView ArticlePubMedGoogle Scholar
- McKenna SP, Cook SA, Whalley D, Doward LC, Richards HL, Griffiths CE, Van Assche D: Development of the PSORIQoL, a psoriasis-specific measure of quality of life designed for use in clinical practice and trials. Br J Dermatol 2003, 149: 323–331. 10.1046/j.1365-2133.2003.05492.xView ArticlePubMedGoogle Scholar
- Ashcroft DM, Li Wan Po A, Williams HC, Griffiths CE: Quality of life measures in psoriasis: A critical appraisal of their quality. J Clin Pharm Ther 1998, 23: 391–398. 10.1046/j.1365-2710.1998.00181.xView ArticlePubMedGoogle Scholar
- Both H, Essink-Bot ML, Busschbach J, Nijsten T: Critical review of generic and dermatology-specific health-related quality of life instruments. J Invest Dermatol 2007, 127: 2726–2739. 10.1038/sj.jid.5701142View ArticlePubMedGoogle Scholar
- Bronsard V, Paul C, Prey S, Puzenat E, Gourraud PA, Aractingi S, Aubin F, Bagot M, Cribier B, Joly P, Jullien D, Le Maitre M, Richard-Lallemand MA, Ortonne JP: What are the best outcome measures for assessing quality of life in plaque type psoriasis? A systematic review of the literature. J Eur Acad Dermatol Venereol 2010, 24: 17–22.View ArticlePubMedGoogle Scholar
- Chren MM: Understanding research about quality of life and other health outcomes. J Cutan Med Surg 1999, 3: 312–316.PubMedGoogle Scholar
- Lewis VJ, Finlay AY: Two decades experience of the psoriasis disability index. Dermatology 2004, 210: 261–268.View ArticleGoogle Scholar
- Shek D, Chan Y, Lee P: Quality of life in the global context: a chinese response. Soc Indic Res 2005, 71: 1–10. 10.1007/s11205-004-8035-xView ArticleGoogle Scholar
- Basra MKA, Fenech R, Gatt RM, Salek MS, Finlay AY: The Dermatology Life Quality Index 1994–2007: a comprehensive review of validation data and clinical results. Br J Dermatol 2008, 159: 997–1035.PubMedGoogle Scholar
- Department of Dermatology and Wound Healing of Cardiff University. Quality of Life & Dermatology Life Quality Index; 2010. Available at: http://www.dermatology.org.uk
- He Z, Lu C, Basra MKA, Ou A, Yan Y, Li L: Psychometric properties of the Chinese version of Dermatology Life Quality Index (DLQI) in 851 Chinese patients with psoriasis. J Eur Acad Dermatol Venereol 2011. 10.1111/j.1468-3083.2011.04371.xGoogle Scholar
- Ware JE, Snow KK, Kosinski MK, Gandek B: SF-36 Health Survey: Manual and Interpretation Guide. Boston: The Health Institute, New England Medical Center; 1993.Google Scholar
- Li L, Wang HM, Shen Y: Chinese SF-36 health survey: translation, cultural adaptation, validation, and normalisation. J Epidemiol Community Health 2003, 57: 259–263. 10.1136/jech.57.4.259PubMed CentralView ArticlePubMedGoogle Scholar
- Schmitt J, Wozel G: The psoriasis area and severity index is the adequate criterion to define severity in chronic plaque-type psoriasis. Dermatology 2005, 210: 194–199. 10.1159/000083509View ArticlePubMedGoogle Scholar
- Anderson RT, Rajagopalan R: Development and validation of a quality of life instrument for cutaneous diseases. J Am Acad Dermatol 1997, 37: 41–50. 10.1016/S0190-9622(97)70210-XView ArticlePubMedGoogle Scholar
- Nijsten T, Whalley D, Gelfand J, Margolis D, McKenna SP, Stern RS: The psychometric properties of the psoriasis disability index in United States patients. J Invest Dermatol 2005, 125: 665–672. 10.1111/j.0022-202X.2005.23775.xView ArticlePubMedGoogle Scholar
- Abramson JH: Survey methods in community medicine. Edinburgh: Churchill Livingstone; 1990.Google Scholar
- Colton T: Statistics in medicine. Boston: Little, Brown; 1974.Google Scholar
- Wahl AK, Wiklund I, Moum T, Hanestad BR: The norwegian version of the psoriasis disability index–a validation and reliability study. Value Health 1999, 2: 342–349. 10.1046/j.1524-4733.1999.25002.xView ArticlePubMedGoogle Scholar
- Aghaei S, Moradi A, Ardekani GS: Impact of psoriasis on quality of life in Iran. Indian J Dermatol Venereol Leprol 2009, 75: 220.View ArticlePubMedGoogle Scholar
- Kent G, al Abadie M: The Psoriasis Disability Index-further analyses. Clin Exp Dermatol 1993, 18: 414–416. 10.1111/j.1365-2230.1993.tb02239.xView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.